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Molecular TILLING and EcoTILLING. Banik et al. 2001; Till et al. 2003a, 2003b; Comai et al. 2004; Henikoff et al. 2004; Till et al. 2004a, 2004b; Gilchrist et al. 2006; Till et al. 2006, 2007; Weil and Monde 2007). mutations, they self fertilized M1s to produce M2 lines for MUTANT GENERATION FOR TILLING
We describe Targeting-Induced Local Lesions IN Genomes (TILLING), a reverse-genetic strategy for the discovery and mapping of induced mutations. TILLING is suitable for essentially any organism
We review here current TILLING and EcoTILLING technologies and discuss the progress that has been made in applying these methods to many different plant species. 1. INTRODUCTION Nucleotide sequence variation is a major determinant of heritable phenotypic difference and has been exploited by humans for crop improvement since the dawn of domestication. Variation can either be natural, from TILLING is suitable for essentially any organism that can be mutagenized. The TILLING procedure has also been adapted for the discovery and cataloguing of natural polymorphisms, a method called Ecotilling. To discover nucleotide changes within a particular gene, PCR is performed with gene-specific primers that are end-labeled with fluorescent
SCENC REPORTS 629250 DI 10.1038srep29250 1 nature.comscientificreports EcoTILLING revealed SNPs in GhSus genes that are associated with fiber- and seed-related traits in upland cotton Yan-Da Zeng1, Jun-Ling Sun2, Su-Hong Bu1, Kang-Sheng Deng1, Tao Tao1, Yuan-Ming Zhang3, Tian-Zhen Zhang1, Xiong-Ming Du2 & Bao-Liang Zhou1 Cotton is the most important textile crop in the world due to its